Abstract:
Nucleotide sequence and polymerase chain reaction (PCR) - restriction fragment length polymorphism
(RFLP) analysis of the ribosomal RNA gene (rDNA) regions containing the internal transcribed spacers (ITSs) and
the 5.8S rRNA coding sequence was used to differentiate between 7 typical Flammulina strains. These nucleotide
sequences revealed the presence of strain-specific deletions, insertions, and substitutions. Moreover, RFLP patterns
produced using restriction endonucleases DraI, FokI, HaeII, MboII, and NlaIV, enabled identification of specific
Flammulina strains. Thus, PCR-RFLP analysis of the ITS regions appears to be a useful tool for the identification of
Flammulina strains.
