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Bioassay guided isolation and identification of the antioxidant constituent from Holostemma Ada-kodien shcult

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dc.contributor.author Sunil, Junapudi
dc.contributor.author Janapati, Yasodha Krishna
dc.contributor.author Bramhachari, Pallaval Veera
dc.date.accessioned 2020-01-03T11:35:16Z
dc.date.available 2020-01-03T11:35:16Z
dc.date.issued 2017
dc.identifier.uri http://erepo.usiu.ac.ke/11732/5152
dc.description Journal article Full text: http://dx.doi.org/10.22376/ijpbs.2017.8.4.p1-10 en_US
dc.description.abstract Holostemma ada-kodien shcult (Syn: Holostemma annulare) is a traditionally used in Indian herbal medicine. The objective of this study is to investigate the antioxidant activity of a bioassay-guided fractionation and its active components/compounds. Compounds were isolated by high performance thin layer chromatography (HPTLC) and preparative high performance liquid chromatography (pre-HPLC) and their structures were established by mass spectrometry (MS), Nuclear magnetic resonance (NMR) and fourier transform infrared spectroscopy (FT-IR) spectroscopic analyses. The antioxidant activity of ethyl acetate extract of Holostemma ada-kodien shcult and its fractions (CE, CF, EAF, MF and AF) were investigated using free radical scavenging activity, superoxide radical-scavenging assay, hydroxyl radical scavenging assay and assay of FeCl3 power. The results revealed that the antioxidant activity of different fractionations such as CE and EAF showed prominent activity when compared with butylated hydroxyltoluene (BHT) in a dose-dependent manner. Two compounds were isolated from Fraction –D among them quercetin and acacetin were reported for the first time in H. ada-kodien. Quercetin showed a more prominent antioxidant activity potential at concentrations (10, 20, 30, 40 and 50μg/mL) when compared with the other compounds. In conclusion the ethyl acetate fraction proved to have significant therapeutic potential for antioxidant effect. en_US
dc.publisher International Jounal of Pharma and Bio Sciences en_US
dc.subject Antioxidant activity, Holostemma ada-kodien shcult, Butylated Hydroxyltoluene, Quercetin, Acacetin en_US
dc.title Bioassay guided isolation and identification of the antioxidant constituent from Holostemma Ada-kodien shcult en_US
dc.type Article en_US


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