Molecular Characterisation of Vibrio Cholerae O1 Strains Carrying an SXT/R391-Like Element from Cholera Outbreaks in Kenya: 1994-2007

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dc.contributor.author Kiiru, John N.
dc.contributor.author Saidi, Suleiman M.
dc.contributor.author Goddeeris, Bruno M.
dc.contributor.author Wamae, C.N.
dc.contributor.author Butaye, Patrick
dc.contributor.author Kariuki, Samuel M.
dc.date.accessioned 2018-06-25T11:32:06Z
dc.date.available 2018-06-25T11:32:06Z
dc.date.issued 2009
dc.identifier.uri http://erepo.usiu.ac.ke/11732/3869
dc.description Journal Article en_US
dc.description.abstract Over the last decade, cholera outbreaks in parts of Kenya have become common. Although a number of recent studies describe the epidemiology of cholera in Kenya, there is paucity of information concerning the diversity and occurrence of mobile genetic elements in Vibrio cholerae strains implicated in these outbreaks. A total of 65 Vibrio cholerae O1 El Tor serotype Inaba isolated between 1994 and 2007 from various outbreaks in Kenya were investigated for mobile genetic elements including integrons, transposons, the integrating conjugative elements (ICEs), conjugative plasmids and for their genotypic relatedness. All the strains were haemolytic on 5% sheep blood and positive for the Vibrio cholerae El Torspecific haemolysin toxin gene (hylA) by PCR. They all contained strB, sulII, floR and the dfrA1 genes encoding resistance to streptomycin, sulfamethoxazole, chloramphenicol and trimethoprim respectively. These genes, together with an ICE belonging to the SXT/R391 family were transferable to the rifampicin-resistant E. coli C600 en bloc. All the strains were negative for integron class 1, 2 and 3 and for transposase gene of transposon Tn7 but were positive for integron class 4 and the trpM gene of transposon Tn21. No plasmids were isolated from any of the 65 strains. All the strains were also positive for all V. cholera El Tor pathogenic genes except the NAG- specific heat-stable toxin (st) gene. None of the strains were positive for virulence genes associated with the V. cholerae classical biotype. All the strains were positive for El Torspecific CTXphi bacteriophage rstrR repressor gene (CTXETΦ) but negative for the Classical, Calcutta, and the Environmental repressor types. Pulse Field Gel Electrophoresis (PFGE) showed that regardless of the year of isolation, all the strains bearing the SXT element were clonally related. This study demonstrates that the V. cholerae O1 strains carrying an SXT/R391-like element implicated in recent cholera outbreaks in Kenya has not changed significantly between 1994 and 2007 and are clonally related. en_US
dc.language.iso en en_US
dc.publisher BioMed Central Ltd. en_US
dc.relation.ispartofseries BMC Microbiology 2009, 9:275;
dc.subject Molecular Characterisation en_US
dc.subject Vibrio Cholerae en_US
dc.subject O1 Strains en_US
dc.subject SXT/R391-Like Element en_US
dc.subject Cholera Outbreaks en_US
dc.subject Kenya en_US
dc.subject 1994 en_US
dc.subject 2007 en_US
dc.title Molecular Characterisation of Vibrio Cholerae O1 Strains Carrying an SXT/R391-Like Element from Cholera Outbreaks in Kenya: 1994-2007 en_US
dc.type Article en_US

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