Spectroelectrochemical Characterization of Pain Biomarkers

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dc.contributor.author Naumih, Noah
dc.contributor.author Omole, Marcells A.
dc.contributor.author Zhou, Lisa
dc.contributor.author Almaletti, Anas
dc.contributor.author Omowunmi, A. Sadik
dc.contributor.author Asemota, Helen N.
dc.contributor.author Elvira, S. William
dc.contributor.author Gilchrist, Jason
dc.date.accessioned 2015-09-30T10:18:58Z
dc.date.available 2015-09-30T10:18:58Z
dc.date.issued 2009
dc.identifier.issn 0003-2697
dc.identifier.issn 1096-0309
dc.identifier.uri http://erepo.usiu.ac.ke/11732/1165
dc.description A journal article by Dr. Naumih M. Noah a lecturer at United States International University - Africa. en_US
dc.description.abstract This article reports the first electrochemical characterization of pain biomarkers that include arachidonic acid (AA), prostaglandin G2 (PGG2), and cyclooxygenase 2 (COX-2). These biomarkers are mediators of pathophysiology of pain, inflammation, and cell proliferation in cancer. The article also reports the development of an electrochemical immunosensor for monitoring these pain biomarkers. The results revealed that direct electron transfer between AA metabolites and the electrode could be easily monitored and that an enzyme-modified electrode dramatically enhanced bioelectrocatalytic activity toward AA. Cyclic voltammetric analysis of AA revealed a concentration-dependent anodic current with a slope of 2.37 and a limit of detection (LOD) of 0.25 nM. This unique AA/gold electrode electron transfer provides a good electrochemical sensing platform for prostaglandin H2 (PGH2) as the basis for quantitation of pain. An amperometric signal intensity of a COX-2 antibody-modified gold electrode was linear with COX-2 concentration in the range of 0.1–0.5 lg/ml and an LOD of 0.095 lg/ml. The results also revealed a linear correlation of the concentration of PGG2 with an LOD of 0.227 lM. en_US
dc.publisher Analytical Biochemistry en_US
dc.subject Pain en_US
dc.subject Cyclic voltammetry en_US
dc.subject Arachidonic acid en_US
dc.subject COX-2 en_US
dc.subject Prostaglandin G2 en_US
dc.title Spectroelectrochemical Characterization of Pain Biomarkers en_US
dc.type Article en_US

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