Characterisation of Species and Diversity of Anopheles gambiae Keele Colony

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dc.contributor.author Ranford-Cartwright, Lisa
dc.contributor.author McGeechan, Sion
dc.contributor.author Inch, Donald
dc.contributor.author Smart, Graeme
dc.contributor.author Richterova, Lenka
dc.contributor.author Mwangi, Jonathan
dc.date.accessioned 2019-08-09T09:42:22Z
dc.date.available 2019-08-09T09:42:22Z
dc.date.issued 2016-12-29
dc.identifier.uri http://erepo.usiu.ac.ke/11732/4733
dc.description Journal Article en_US
dc.description.abstract Anopheles gambiae sensu stricto was recently reclassified as two species, An. coluzzii and An. gambiae s.s., in wild-caught mosquitoes, on the basis of the molecular form, denoted M or S, of a marker on the X chromosome. The An. gambiae Keele line is an outbred laboratory colony strain that was developed around 12 years ago by crosses between mosquitoes from 4 existing An. gambiae colonies. Laboratory colonies of mosquitoes often have limited genetic diversity because of small starting populations (founder effect) and subsequent fluctuations in colony size. Here we describe the characterisation of the chromosomal form(s) present in the Keele line, and investigate the diversity present in the colony using microsatellite markers on chromosome 3. We also characterise the large 2La inversion on chromosome 2. The results indicate that only the M-form of the chromosome X marker is present in the Keele colony, which was unexpected given that 3 of the 4 parent colonies were probably S-form. Levels of diversity were relatively high, as indicated by a mean number of microsatellite alleles of 6.25 across 4 microsatellites, in at least 25 mosquitoes. Both karyotypes of the inversion on chromosome 2 (2La/2L+a) were found to be present at approximately equal proportions. The Keele colony has a mixed M- and S-form origin, and in common with the PEST strain, we propose continuing to denote it as an An. gambiae s.s. line. en_US
dc.description.sponsorship his work was supported by the Wellcome Trust: 091791 (LRC, JMM), 089886/Z/09/Z (LRC, LR) [wellcome.ac.uk]. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. en_US
dc.publisher PLOS ONE en_US
dc.subject AnophelesgambiaeKeeleColony en_US
dc.title Characterisation of Species and Diversity of Anopheles gambiae Keele Colony en_US
dc.type Article en_US

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